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Anti-DMRTA2 Antibody
Catalog Number: orb1879874
| Catalog Number | orb1879874 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to DMRTA2 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Zebrafish |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human DMRTA2. The exact sequence is proprietary. |
| UniProt ID | A2A9A2, Q96SC8 |
| Tested applications | IF, WB |
| Dilution range | WB (1/500 - 1/1000), IF/IC (1/10 - 1/50) |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | DMRT5; Doublesex- and mab-3-related transcription Read more... |
| Note | For research use only |
| Entrez | 63950, 242620 |
Western blot analysis of DMRTA2 expression in PC3 (A), mouse testis (B), zebrafish brain (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 53 kD)
Immunofluorescent analysis of Anti-DMRTA2 staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
