Anti-Disialoganglioside 2 [14G2a]

• Catalog Number: orb1906419
• Category: Antibodies
• Description: Anti-Disialoganglioside 2 [14G2a]
• Target: Disialoganglioside 2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3).
• Tested applications: ELISA, FC
• Clone Number: 14G2a
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GD2
• Note: For research use only

Flow cytometry using anti-Disialoganglioside 2 antibody 14G2a. Kelly cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 14G2a (orb1906418, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Kelly cells with anti-Disialoganglioside 2 antibody 14G2a. Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14G2a (orb1906418) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906418, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.