Anti-desmosome/hemidesmosome [F12]

• Catalog Number: orb613932
• Category: Antibodies
• Description: Human monoclonal antibody to desmosome
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: F12
• Tested applications: IEM, IF
• Reactivity: Human
• Isotype: IgM
• Immunogen: F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: desmosome/hemidesmosome
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-desmosome/hemidesmosome antibody F12 A431 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of F12 (orb613933, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.