Dendra2 Antibody

SKU: orb699857

Description

Rabbit polyclonalantibody to Dendra2

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, WB
Reactivity	All
Application Notes	Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml. Extracellular and intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Polyclonal
Isotype	Rabbit polyclonal
Clone No.	PAb (836)
Immunogen	Dendra2 tag
Target	Dendra2
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Dendra2 tag

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Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct; reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of anti-Dendra2 rabbit polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for Dendra2/GPI fusion protein at approximately 35 kDa.

Quick Database Links

Gene Symbol

Dendra2

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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