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Anti-DDX5 Antibody

Catalog Number: orb256492

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb256492
CategoryAntibodies
DescriptionRabbit polyclonal antibody to DDX5
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Primate, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DDX5. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetDDX5
Entrez1655
UniProt IDP17844, Q61656
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti G17P1 antibody, anti HELR antibody, anti HLR1
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-DDX5 Antibody

Western blot analysis of DDX5 expression in Hela (A), H446 (B) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 68 kD)

Anti-DDX5 Antibody

Immunofluorescent analysis of DDX5 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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