Anti-DDX3X Antibody

• Catalog Number: orb1474289
• Category: Antibodies
• Description: Mouse monoclonal antibody to DDX3X
• Target: DDX3X
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Primate, Rat
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Purified recombinant human DDX3 protein fragments expressed in E.coli.
• UniProt ID: Q62167, O00571
• Tested applications: ChIP, IF, IP, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD
• Note: For research use only
• Entrez: 1654, 13205

Western blot analysis of DDX3X expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), K562 (E), Jurkat (F) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 73 kD)

Immunofluorescent analysis of DDX3X staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.