DDAH1 Monoclonal Antibody

SKU: orb548168

Description

Anti-DDAH1 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200IP 1:40FC 1:30. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AEAG-4
• Immunogen: A synthesized peptide derived from human DDAH1 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
• Molecular Weight: 37 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; CLH-17; CLTC; CLH17, CLTCL2, KIAA0034

Western blot analysis of DDAH1 expression in 293 cell lysate using anti-DDAH1 antibody (orb548168). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified polyclonal antibody (Catalog # orb548168) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for DDAH1.

Quick Database Links

UniProt

O94760