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Anti-DCP1A Antibody

Catalog Number: orb338893

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb338893
CategoryAntibodies
DescriptionRabbit polyclonal antibody to DCP1A
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human DCP1A. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetDCP1A
Entrez55802, 75901
UniProt IDQ91YD3, Q9NPI6
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti SMIF antibody, anti mRNA-decapping enzyme 1A
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-DCP1A Antibody

Western blot analysis of DCP1A expression in HuvEc (A), Jurkat (B) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 63 kD)

Anti-DCP1A Antibody

Immunohistochemical analysis of DCP1A staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-DCP1A Antibody

Immunofluorescent analysis of DCP1A staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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