Anti-DCIR [9E8]

• Catalog Number: orb669739
• Category: Antibodies
• Description: Mouse monoclonal antibody to DCIR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9E8
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunising BALB/c mice with a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: DCIR
• UniProt ID: Q9UMR7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD367; Clec4A; C-type lectin domain family 4 membe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-DCIR antibody 9E8. Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of 9E8 (orb669739, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of human peripheral blood monocytes using anti-DCIR antibody 9E8. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of 9E8 (orb669739) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669739, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.