Anti-DAZL Monoclonal Antibody

• Catalog Number: orb547944
• Category: Antibodies
• Description: Anti-DAZL Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: ACBF-4
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human DAZL RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
• Antibody Type: Primary Antibody
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• UniProt ID: Q92904
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Junctional adhesion molecule A; JAM-A; Junctional
• Note: For research use only
• Application notes: WB 1:500-1:2000. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DAZL expression in human testis lysate using anti-DAZL antibody (orb547944). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZL antigen affinity purified polyclonal antibody (Catalog # orb547944) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for DAZL.