Anti-DAP Kinase 1 (Phospho-S736) Antibody

• Catalog Number: orb515280
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DAPK1 (phospho-S736)
• Target: DAPK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S736 of human DAP Kinase 1 protein. The exact sequence is proprietary.
• UniProt ID: Q80YE7, P53355
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DAPK; Death-associated protein kinase 1; DAP kinas
• Note: For research use only
• Entrez: 69635, 1612

Western blot analysis of DAP Kinase 1 (Phospho-S736) expression in HEK293T (A), CT26 (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 160 kD)

Immunohistochemical analysis of DAP Kinase 1 (Phospho-S736) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.