Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody

SKU: orb547742

Description

Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB 1:1000-5000IHC 1:50-200ICC/IF 1:50-200IP 1:20FC 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: CIG-11
• Immunogen: A synthesized peptide derived from human Cytokeratin 8
• Target: Glycogen synthase kinase-3 beta (gsk3b)
• Molecular Weight: 40 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8; KRT8; CYK8

Immunohistochemical analysis of paraffin-embedded human liver, using Cytokeratin 8 Antibody (orb547742) KRT8 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KRT8 Antibody (orb547742) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of Cytokeratin 8 expression in HaCaT cell lysate (orb547742). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT8 monoclonal antibody (Catalog # orb547742) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for KRT8.

Quick Database Links

Gene Symbol

Glycogen synthase kinase-3 beta (gsk3b)

UniProt

P05787