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Catalog Number | orb154469 |
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Category | Antibodies |
Description | Mouse monoclonal antibody conjugated to FITC which recognizes Cytokeratin 8 that belongs to the intermediate filament family. It expressed in epithelium and epithelium-derived tumors. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy. |
Target | Cytokeratin 8 |
Clonality | Monoclonal |
Isotype | Mouse IgG1 |
Conjugation | FITC |
Reactivity | Bovine, Human, Porcine, Rabbit, Sheep |
Concentration | 1 mg/ml |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Immunogen | Cytoskeleton preparation from HeLa human cervix carcinoma cell line. |
UniProt ID | Q7L4M3 |
Tested applications | FC |
Application notes | Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining. |
Antibody Type | Primary Antibody |
Clone Number | C-43 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Alternative names | Anti-K8 antibody, anti-CK8 antibody, anti-CYK8 ant Read more... |
Note | For research use only |
Entrez | 3856 |
Separation of A431 cellular suspension (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).
Flow cytometry intracellular staining pattern of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).