Cytokeratin 8 Antibody (FITC)

SKU: orb154469

Description

Mouse monoclonal antibody conjugated to FITC which recognizes Cytokeratin 8 that belongs to the intermediate filament family. It expressed in epithelium and epithelium-derived tumors. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.

Images & Validation

• Tested Applications: FC
• Reactivity: Bovine, Human, Porcine, Rabbit, Sheep
• Application Notes:
  Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: C-43
• Immunogen: Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
• Target: Cytokeratin 8
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

K8, CK8, CYK8, KRT8, K2C8, CARD2

Separation of A431 cellular suspension (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).

Flow cytometry intracellular staining pattern of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).

Quick Database Links

Gene Symbol

Cytokeratin 8

UniProt

Q7L4M3

NCBI Gene ID

3856