Anti-Cytokeratin 18 Purified

• Catalog Number: orb43750
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 18
• Clonality: Monoclonal
• Clone Number: DC-10
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human breast carcinoma cell line PMC-42.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cytokeratin 18
• Entrez: 3875
• UniProt ID: P05783
• RRID: AB_10993301
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-CYK 18 antibody, anti-CYK18 antibody, anti-CK
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100). Flow cytometry: Recommended dilution: 6-16 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells (red-filled) from Caco-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cells stained using anti-cytokeratin 18 (DC-10) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (orb43750, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.