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Cytokeratin 18 Antibody

SKU: orb43702

Description

Mouse monoclonal antibody to Cytokeratin 18

Research Area

Epigenetics, Infectious Diseases

Images & Validation

Tested ApplicationsELISA, FC, ICC, IHC-P, IP, WB
ReactivityMammal
Application Notes
Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive control: colon, kidney. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of cytokeratin 18 in combination with the antibody DA-7 (cat. no. orb43747) and with the antibody DC-10 (cat. no. orb43751). Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.C-04
ImmunogenCytoskeleton preparation of epidermal carcinoma cell line A431.
TargetCytokeratin 18
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

K18, CK18, CYK18, KRT18

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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0.1 mg
$ 300.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days.
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