Cytochrome P450 4F3 Antibody

• Catalog Number: orb1879474
• Category: Antibodies
• Description: The Cytochrome P450 4F3 Antibody is suitable for FC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human Cytochrome P450 4F3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human Cytochrome P450 4F3. The exact sequence is proprietary.
• UniProt ID: Q08477
• Tested applications: FC, WB
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LTB4H; Leukotriene-B(4) omega-hydroxylase 2; CYPIV
• Note: For research use only
• Entrez: 4051

Western blot analysis of Cytochrome P450 4F3 expression in K562 (A) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 63 kD)

Flow cytometric analysis of HepG2 cells using Anti-Cytochrome P450 4F3 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.