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Item 1 of 2
Anti-Cyclin D3 (Phospho-T283) Antibody
Catalog Number: orb338829
| Catalog Number | orb338829 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to CCND3 |
| Target | CCND3 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T283 of human Cyclin D3 protein. The exact sequence is proprietary. |
| UniProt ID | P48961, P30282, P30281 |
| Tested applications | IH, WB |
| Dilution range | WB: 1:500-1000, IHC-P: 1:100-200 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti G1/S-specific cyclin-D3 antibody |
| Note | For research use only |
| Entrez | 896, 25193, 12445 |
Western blot analysis of Cyclin D3 (Phospho-T283) expression in HepG2 UV-treated (A), K562 UV-treated (B) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 32 kD)
Immunohistochemical analysis of Cyclin D3 (Phospho-T283) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
