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Cyclin D1 Antibody (Biotin)

SKU: orb1562576

Description

Cyclin D1 Antibody (Biotin)

Images & Validation

Tested ApplicationsFC, ICC, IHC-Fr, IHC-P, IP, WB
ReactivityHuman, Mouse, Primate, Rat
Application Notes
Flow cytometry: Recommended dilution: 2-10 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 5-10 μg/ml.

Key Properties

ClonalityMonoclonal
IsotypeMouse IgG2a
Clone No.DCS-6
Immunogenrecombinant human cyclin D1 (amino acids 1-295)
TargetCyclin D1
PurificationPurified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
ConjugationBiotin

Storage & Handling

StorageStore at 2-8°C. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

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Cyclin D1 Antibody (Biotin)

Separation of MCF-7 cells stained using anti-Cyclin D1 (DCS-6) Biotin antibody (concentration in sample 5.0 μg/ml, Streptavidin APC, red-filled) from MCF-7 cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (intracellular staining).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
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IHC-Fr
Immunohistochemistry Frozen
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FC
Flow Cytometry
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ICC
Immunocytochemistry
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IP
Immunoprecipitation
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Cyclin D1 Antibody (Biotin) (orb1562576)

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0.1 mg
$ 350.00
DispatchUsually dispatched within 5-10 working days
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