Anti-CTLA-4 (ipilimumab biosimilar) mAb

• Catalog Number: orb689327
• Category: Antibodies
• Description: Anti-CTLA4 (ipilimumab biosimilar) mAb
• Target: CTLA-4
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P16410
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000|Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CTLA4;CD152
• Note: For research use only

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CTLA4, mFc-His tagged protein ([getskuurl sku"orb689378"]) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.13-16.0 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CTLA4, hFc tagged protein (orb689463) can bind Anti-CTLA4 Neutralizing antibody in a linear range of 0.64-80.0 ng/ml. In order to specifically detect orb689327, mouse anti-human Fab-specific antibody was used as detection antibody.

HEK293 cell line transfected with irrelevant protein (B) and human CTLA4 (A) were surface stained with anti-CTLA4 neutralizing antibody 1 μg/ml (ipilimumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.