Anti-CTLA-4 [9H10]

SKU: orb758786

Description

Rabbit monoclonal antibody to CTLA4

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Mouse

Key Properties

• Host: Hamster
• Clonality: Monoclonal
• Isotype: Hamster IgG
• Clone No.: 9H10
• Immunogen: This antibody was raised by immunising Syrian hamsters with Staphylococcus A bacteria coated in CTLA-4, isolating B cells from the immunised hamsters and fusing these with the P3X3.Ag8.653 myeloma line to produce stable hybridomas.
• Target: CTLA-4
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD152; CTLA4; cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte protein 4;

ELISA of anti-CTLA-4 antibody on mouse CTLA-4-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-CTLA-4 antibody 9H10 (orb758786; red line) and isotype control (anti-Fluorescein antibody orb256390 4-4-20 (enhanced); blue line) to an ELISA plate coated with mouse CTLA-4-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using orb758786. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.

Quick Database Links

Gene Symbol

CTLA-4

UniProt

P09793