Anti-CRP [P24]

SKU: orb1463342

Description

Mouse monoclonal antibody to CRP

Research Area

Immunology & Inflammation, Microbiology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: P24
• Immunogen: The original antibody was generated by immunizing BALB/c mice with C-reactive protein extracted from human plasma.
• Target: CRP
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C reactive protein; PTX1; C-reactive protein

Immunofluorescence staining of HepG2 cells with anti-CRP P24. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of P24 (orb1463341) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463341, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CRP antibody P24. Human liver(A) (0.0003 µg/ml), kidney(B) (0.0003 µg/ml), staining in human plasma(C) (0.001 µg/ml) and staining in human serum(D) (0.01 µg/ml) tissue lysate (35 µg protein in RIPA buffer) wer resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of P24 (orb1463341) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

CRP

UniProt

P02741