Anti-CREB (Phospho-S133) Antibody

• Catalog Number: orb1474549
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CREB (phospho-S133)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: CREB1
• Entrez: 81646, 1385
• UniProt ID: P15337, P16220
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: Cyclic AMP-responsive element-binding protein 1; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CREB (Phospho-S133) expression in C6 UV treated (A) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)

Immunohistochemical analysis of CREB (Phospho-S133) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.79). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.