Anti-CREB (Phospho-S133) Antibody

• Catalog Number: orb1474549
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CREB (phospho-S133)
• Target: CREB1
• Clonality: Monoclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB protein. The exact sequence is proprietary.
• UniProt ID: P16220, P15337
• Tested applications: IH, IP, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cyclic AMP-responsive element-binding protein 1; C
• Note: For research use only
• Entrez: 81646, 1385

Western blot analysis of CREB (Phospho-S133) expression in C6 UV treated (A) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)

Immunohistochemical analysis of CREB (Phospho-S133) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.79). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.