Anti-COX IV COX4I1 Rabbit Monoclonal Antibody

• Catalog Number: orb548370
• Category: Antibodies
• Description: Anti-COX IV COX4I1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: AIC-3
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human COX IV
• Antibody Type: Primary Antibody
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• UniProt ID: P13073
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cytochrome c oxidase subunit 4 isoform 1, mitochon
• Note: For research use only
• Application notes: WB 1:1000-1:2000IHC 1:50-1:200ICC/IF 1:100-1:500IP 1:20FC 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Expiration Date: 12 months from date of receipt.

Western blot analysis of COX IV expression in HepG2 cell lysate (orb548370). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX4I1 monoclonal antibody (Catalog # orb548370) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for COX4I1.