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Anti-Connexin 46 Antibody

Catalog Number: orb256559

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb256559
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Connexin 46
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human Connexin 46. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetGJA3
Entrez79217, 2700, 14611
UniProt IDQ64448, P29414, Q9Y6H8
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Gap junction alpha-3 protein antibody, anti C
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Connexin 46 Antibody

Western blot analysis of Connexin 46 expression in A549 (A), U2OS (B), DLD (C), mouse heart (D), rat heart (E) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 53 kD)

Anti-Connexin 46 Antibody

Immunohistochemical analysis of Connexin 46 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Connexin 46 Antibody

Immunofluorescent analysis of Connexin 46 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

  • Anti-Connexin 46 Antibody [orb1422795]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Connexin 46 rabbit pAb [orb768402]

    ELISA,  IHC-P

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • GJA3 Antibody [orb673572]

    ELISA,  IHC

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg
  • Connexin 46 peptide [orb256849]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg
  • GJA3 polyclonal antibody [orb752001]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl