Anti-Complement Factor I [OX-21]

• Catalog Number: orb2645707
• Category: Antibodies
• Description: Anti-Complement Factor I [OX-21]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OX-21
• Tested applications: ELISA, FC, IHC, IHC-P, IP, RIA, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: C3bINA was purified from outdated human plasma, and subcutaneously injected into DBA/2 x Balb/c F1 mice.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: Complement Factor I
• UniProt ID: P05156
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: C3b/C4b inactivator; CFI; AHUS3; ARMD13; C3BINA; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-Complement Factor I antibody OX-21. Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of OX-21 (orb2645706, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.