Cart summary

You have no items in your shopping cart.

Anti-Collagen 6 alpha 2 Antibody

Catalog Number: orb213763

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb213763
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Collagen VI
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Primate, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 6 alpha 2. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetCOL6A2
Entrez1292
UniProt IDP12110
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti COL6A1 antibody, anti Collagen alpha-1(VI) ch
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Collagen 6 alpha 2 Antibody

Western blot analysis of Collagen 6 alpha 2 expression in Hela (A), U2OS (B), mouse lung (C), mouse kidney (D), rat lung (E), rat kidney (F) whole cell lysates. (Predicted band size: 108 kD; Observed band size: 97; 87 kD)

Anti-Collagen 6 alpha 2 Antibody

Immunohistochemical analysis of Collagen 6 alpha 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Collagen 6 alpha 2 Antibody

Immunofluorescent analysis of Collagen 6 alpha 2 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Collagen 6 alpha 2 Antibody [orb652984]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Collagen VI peptide [orb217119]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg