Collagen 3 alpha 1 Mouse Monoclonal Antibody

SKU: orb323123

Description

The Collagen 3 alpha 1 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human Collagen 3 alpha 1. The exact sequence is proprietary. Purification: Affinity chromatography

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Collagen 3 alpha 1. The exact sequence is proprietary.
• Target: COL3A1
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen alpha-1(III) chain

Western blot analysis of Collagen 3 alpha 1 expression in Hela (A) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 138 kD)

Immunohistochemical analysis of Collagen 3 alpha 1 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

COL3A1

UniProt

P02461, P13941, P08121

NCBI Gene ID

1281, 84032, 12825