Anti-Collagen 17 alpha 1 Antibody

• Catalog Number: orb382697
• Category: Antibodies
• Description: Rabbit polyclonal antibody to COL17A1
• Target: COL17A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 17 alpha 1. The exact sequence is proprietary.
• UniProt ID: Q07563, Q9UMD9
• Tested applications: IH, WB
• Dilution range: WB: 1:500:2000, IHC-P: 1:50:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-BP180 antibody, anti-BPAG2 antibody, anti-Col
• Note: For research use only
• Entrez: 1308, 12821

Western blot analysis of Collagen 17 alpha 1 expression in rat kidney (A), HCT116 (B), HepG2 (C), 3T3L1 (D) whole cell lysates. (Predicted band size: 150 kD; Observed band size: 130 kD)

Immunohistochemical analysis of Collagen 17 alpha 1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.