Anti-Collagen 15 alpha 1 Antibody

• Catalog Number: orb234811
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen 15 alpha 1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Primate
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Collagen 15 alpha 1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: COL15A1
• Entrez: 1306, 12819
• UniProt ID: P39059, O35206
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Collagen alpha-1(XV) chain antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Collagen 15 alpha 1 expression in HEK293T (A) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 141 kD)

Immunofluorescent analysis of Collagen 15 alpha 1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).