Anti-CMKLR1 [BZ194]

• Catalog Number: orb758808
• Category: Antibodies
• Description: Rat monoclonal antibody to CMKLR1
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: BZ194
• Tested applications: ELISA, FC, Functional Studies, IF, IHC, IP, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mCMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8–32 of mCMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CMKLR1
• UniProt ID: P97468
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Chemerin receptor; CHEMERINR; chemokine receptor-l
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CMKLR1 antibody BZ194. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BZ194 (orb758807) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758807, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.