Anti-Claudin 2 Antibody

• Catalog Number: orb214855
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CLDN2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 2. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CLDN2
• Entrez: 12738, 9075
• UniProt ID: O88552, P57739
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Claudin-2 antibody, anti SP82 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Anella Saviano 1, Anna Schettino 1, Nunzia Iaccarino 2, Adel Abo Mansour 3, Jenefa Begum 4, Noemi Marigliano 1, Federica Raucci 1, Francesca Romano 2, Gelsomina Riccardi 2, Emma Mitidieri 2, Roberta d'Emmanuele di Villa Bianca 2, Ivana Bello 2, Elisabetta Panza 2, Martina Smimmo 2, Valentina Vellecco 2, Peter Rimmer 5, Jonathan Cheesbrough 6, Zhaogong Zhi 4, Tariq H Iqbal 7, Stefano Pieretti 8, Vincenzo Maria D'Amore 2, Luciana Marinelli 2, Valeria La Pietra 2, Raffaella Sorrentino 2, Luisa Costa 9, Francesco Caso 9, Raffaele Scarpa 9, Giuseppe Cirino 2, Antonio Randazzo 2, Mariarosaria Bucci 2, Helen Michelle McGettrick 10, Asif Jilani Iqbal 11, Francesco Maione A reverse translational approach reveals the protective roles of Mangifera indica in inflammatory bowel disease J Autoimmun, (2024)

PubMed: 38522129

Applications

WB

Reactivity

Mouse

Western blot analysis of Claudin 2 expression in AML12 (A) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)

Immunohistochemical analysis of Claudin 2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Claudin 2 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).