Claudin 2 Rabbit Polyclonal Antibody

SKU: orb214855

Description

The Claudin 2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 2. The exact sequence is proprietary.
• Target: CLDN2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Claudin-2; SP82

Western blot analysis of Claudin 2 expression in AML12 (A) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)

Immunohistochemical analysis of Claudin 2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Claudin 2 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

CLDN2

UniProt

O88552, P57739

NCBI Gene ID

12738, 9075

Anella Saviano 1, Anna Schettino 1, Nunzia Iaccarino 2, Adel Abo Mansour 3, Jenefa Begum 4, Noemi Marigliano 1, Federica Raucci 1, Francesca Romano 2, Gelsomina Riccardi 2, Emma Mitidieri 2, Roberta d'Emmanuele di Villa Bianca 2, Ivana Bello 2, Elisabetta Panza 2, Martina Smimmo 2, Valentina Vellecco 2, Peter Rimmer 5, Jonathan Cheesbrough 6, Zhaogong Zhi 4, Tariq H Iqbal 7, Stefano Pieretti 8, Vincenzo Maria D'Amore 2, Luciana Marinelli 2, Valeria La Pietra 2, Raffaella Sorrentino 2, Luisa Costa 9, Francesco Caso 9, Raffaele Scarpa 9, Giuseppe Cirino 2, Antonio Randazzo 2, Mariarosaria Bucci 2, Helen Michelle McGettrick 10, Asif Jilani Iqbal 11, Francesco Maione A reverse translational approach reveals the protective roles of Mangifera indica in inflammatory bowel disease J Autoimmun, (2024)

PubMed: 38522129

Applications

WB

Reactivity

Mouse