Anti-CK1 delta Antibody

• Catalog Number: orb213788
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSNK1D
• Target: CSNK1D
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CK1 delta. The exact sequence is proprietary.
• UniProt ID: Q06486, Q9DC28, P48730
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HCKID antibody, anti CKI-delta antibody, anti
• Note: For research use only
• Entrez: 104318, 1453, 64462

Western blot analysis of CK1 delta expression in HEK293T (A), Hela (B), H1688 (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunofluorescent analysis of CK1 delta staining in BV2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).