Anti-Cingulin Antibody

• Catalog Number: orb666280
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cingulin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human Cingulin. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CGN
• Entrez: 57530
• UniProt ID: Q9P2M7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-KIAA1319 antibody, anti-Cingulin antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Cingulin expression in LO2 (A), A549 (B) whole cell lysates. (Predicted band size: 87; 136 kD; Observed band size: 160 kD)

Immunohistochemical analysis of Cingulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.