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Anti-CHRM2 Antibody

Catalog Number: orb665625

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb665625
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CHRM2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Porcine, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHRM2. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1000, IF: 1-100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetCHRM2
Entrez1129, 81645, 243764, 102551851
UniProt IDP08172, P10980, Q9ERZ4
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-Muscarinic acetylcholine receptor M2 antibody
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CHRM2 Antibody

Western blot analysis of CHRM2 expression in HEK293T (A), mouse lung (B), mouse kidney (C), rat kidney (D) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52; 70 kD)

Anti-CHRM2 Antibody

Immunofluorescent analysis of CHRM2 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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