Anti-CDX2 [12B6]

SKU: orb1821538

Description

Anti-CDX2 [12B6]

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: 12B6
• Immunogen: The original antibody was generated by immunizing a mouse with the CDX2-KLH protein.
• Target: CDX2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2; rabbit mono-ERP2764Y; TA347

Flow cytometry using anti-CDX2 antibody 12B6. Paraformaldehyde fixed Caco-2 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 12B6 (orb1821538, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Caco-2 cells with anti-CDX2 antibody 12B6. Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 12B6 (orb1821538) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821538, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-CDX2 antibody 12B6. Caco-2 (A) (0.003 µg/ml) cells lysate and human colorectal carcinoma (B) (0.003 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 12B6 (orb1821538) at the mentioned respective concentration before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

CDX2

UniProt

Q99626