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Catalog Number | orb1806273 |
---|---|
Category | Antibodies |
Description | Anti-CDH6 / K-Cadherin Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa. |
Clonality | Monoclonal |
Tested applications | ELISA, FA, FACS, Kinetics |
Reactivity | Human |
Isotype | IgG |
Purity | > 95% |
Conjugation | Unconjugated |
MW | 150 kDa |
Target | CDH6 / K-Cadherin |
Endotoxins | < 1 EU/mg |
Storage | -80°C |
Buffer/Preservatives | 100 mM Pro-Ac 20 mM Arg pH 5.0 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-CDH6 / K-Cadherin Reference Antibody (HKT288)is more than 97.93% ;determined by SEC-HPLC.
Immobilized Cyno CDH6 His at 2 μg/mL can bind Anti-CDH6 / K-Cadherin Reference Antibody (HKT288);EC50=0.006944 μg/mL.
Human CDH6 HEK293(A6) cells were stained with Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC71=0.08624 ug/mL.
The endocytosis ratio HKT288 by Hu-CDH6-HEK293(A6) increased with the increase of antibody concentration; and the Internalization Rate (%);reached 93% at antibody concentration of 2 nM.
HKT288 induced OVCAR3 Luciferase activity was evaluated using huCD16a(V158)-NF-AT-Jurkat Reporter Cell. The EC50 was approximately 0.6792 nM.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human, Monkey | |
Monoclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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