CDH6/K-Cadherin Antibody

SKU: orb1806273

Description

Anti-CDH6 / K-Cadherin Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Clonality: Monoclonal
• Target: CDH6 / K-Cadherin
• Source/Expression System: CHO
• Molecular Weight: 144.24 kDa
• Purity: >95%
• Purification: Protein A
• Endotoxin: <0.001 EU/ug
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Concentration: Batch dependent
• Disclaimer: For research use only

Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CDH6 / K-Cadherin Reference Antibody (HKT288)is more than 97.93% ;determined by SEC-HPLC.

Immobilized Cyno CDH6 His at 2 μg/mL can bind Anti-CDH6 / K-Cadherin Reference Antibody (HKT288);EC50=0.006944 μg/mL.

Human CDH6 HEK293(A6) cells were stained with Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC71=0.08624 ug/mL.

The endocytosis ratio HKT288 by Hu-CDH6-HEK293(A6) increased with the increase of antibody concentration; and the Internalization Rate (%);reached 93% at antibody concentration of 2 nM.

HKT288 induced OVCAR3 Luciferase activity was evaluated using huCD16a(V158)-NF-AT-Jurkat Reporter Cell. The EC50 was approximately 0.6792 nM.

Quick Database Links

Gene Symbol

CDH6 / K-Cadherin

UniProt

P55285