Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)

• Catalog Number: orb1805486
• Category: Antibodies
• Description: Anti-CDH17 / Cadherin-17 Reference Antibody) is expressed from CHO. The heavy chain type is IgG1, and the light chain type is hukappa. It has a predicted MW of 143.68kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 143.68 kDa
• Target: CDH17 / Cadherin-17
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)is more than 98.15% ;determined by SEC-HPLC.

Immobilized human CDH17; His Tag at 2 μg/mL can bind Anti-CDH17 / Cadherin-17 Reference Antibody (10C12);EC50=0.00468μg/mL.

Human CDH17 HEK293 cells were stained with Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC240=0.06889 μg/mL.

The endocytosis ratio 10C12 by Human CDH17 HEK293 increased with the increase of antibody concentration; and the Internalization Rate (%) reached 80% at antibody concentration of 2 nM.

10C12 inhibited the tumor growth of COLO205 on nude mice. The result showed significant anti-tumor effects; with an tumor inhibition rate (TGI) of 70.0% at 5 mpk at D26.