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Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody

Catalog Number: orb548150

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Catalog Numberorb548150
CategoryAntibodies
DescriptionAnti-CDC34/Ubch3 Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityMonoclonal
Clone NumberIBO-3
Tested applicationsFC, ICC, IF, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human CDC34
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW56 kDa
UniProt IDP49427
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesUbiquitin-conjugating enzyme E2 R1; 6.3.2.19; Ubiq
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NoteFor research use only
Application notesWB 1:500-1:2000ICC/IF 1:50-1:200IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody

Western blot analysis of CDC34 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate (orb548150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC34 monoclonal antibody (Catalog # orb548150) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CDC34.