Anti-CD98 [RL388]

• Catalog Number: orb758855
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD98
• Target: CD98
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising rats with an IL 2-producing subline of the mouse T lymphoma EL4.
• UniProt ID: P10852
• Tested applications: FC, IP
• Clone Number: RL388
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 4F2hc; 4F2 cell-surface antigen heavy chain; Solut
• Research Area: Immunology & Inflammation
• Note: For research use only

Immunofluorescence staining of mouse splenocytes using anti-CD98 antibody RL388. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of RL388 (orb758855) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758855, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD98 antibody RL388. Mouse fetal liver lysate (35 µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 (orb758855) at 0.05 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.