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Anti-CD90 Antibody-PE labled

Anti-CD90 Antibody-PE labled

Catalog Number: orb1293697

DispatchUsually dispatched within 5-10 working days
$ 130.00
Catalog Numberorb1293697
CategoryAntibodies
DescriptionRat monoclonal antibody against CD90 conjugated to PE
Species/HostRat
ClonalityMonoclonal
Clone NumberG7
Tested applicationsFC, IF
ReactivityMouse
ImmunogenMouse T-cell hybridoma C6/G8
Antibody TypePrimary Antibody
Dilution range10 μl / assay
Form/AppearanceRat IgG2c kappa. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
ConjugationPE
TargetTHY1
Entrez21838
UniProt IDP01831
SourceRat
StorageShipped and store at 4°C for one year. Do not freeze.
Buffer/PreservativesRat IgG2c kappa. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Alternative namesThy-1 membrane glycoprotein; CDw90; Thy-1 antigen;
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NoteFor research use only
Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Expiration Date12 months from date of receipt.
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