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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, FC, IP |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | 14A8 (14.1) |
| Immunogen | The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen. |
| Target | CD89 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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![Anti-CD89 [14A8 (14.1)]](/images/pub/media/catalog/product/NewWebsite/35/orb1152462_1.png)
Flow cytometry using the Anti-CD89 antibody 14A8 (14.1). Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 14A8 (14.1) (orb1152462, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Protocol Information
Anti-CD89 [14A8 (14.1)] (orb1152462)
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