Anti-CD80 [IDEC-114 (Galiximab)]

• Catalog Number: orb613915
• Category: Antibodies
• Description: Human monoclonal antibody to CD80
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: IDEC-114 (Galiximab)
• Tested applications: Blocking, ELISA, FC, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD80
• UniProt ID: P33681
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B7-1; T-lymphocyte activation antigen CD80; Activa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galiximab). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) (orb613916) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613916, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.