CD8 Mouse Monoclonal Antibody (APC/Cy7)

SKU: orb1294032

Description

The CD8 Antibody-APC/Cy7 labled is suitable for FC, IF. It is a Monoclonal, APC/Cy7 conjugated antibody which raised against Native purified human CD8.. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: 10 μl / assay
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: RFT8
• Immunogen: Native purified human CD8.
• Target: CD8A; CD8B
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: APC/Cy7

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a; CD8B1; T-cell surface glycoprotein CD8 beta chain; CD antigen CD8b

Quick Database Links

Gene Symbol

CD8A; CD8B

UniProt

P10966, P01732

NCBI Gene ID

926, 925