CD79B Biosimilar Antibody

• Catalog Number: orb1826917
• Category: Antibodies
• Description: Anti-CD79B(polatuzumab biosimilar) mAb
• Target: CD79B
• Clonality: Monoclonal
• Species/Host: Human Humanized
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P40259
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000, Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B29IGB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis with 15 μg/mL Anti-CD79B (polatuzumab biosimilar) mAb (orb1826917) on Expi293 cells transfected with Human CD79B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD79B Protein, hFc Tag (orb1173717) can bind Anti-CD79B (polatuzumab biosimilar) mAb (orb1826917) in a linear range of 0.64–16 ng/ml. In order to specifically detect orb1826917, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis of antigen binding of anti-human CD79B mAb (orb1826917). (A) orb1826917 does not bind to 293T cells that do not express CD79B. (B) A clear peak shift of orb1826917 was seen compared to the control when incubated with CD79B-expressing Raji cells, indicating strong binding of orb1826917 to CD79B. Antibodies were incubated at 5 μg/mL.