Anti-CD74 [LL1 (EPB1)]

• Catalog Number: orb1089962
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD74
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LL1 (EPB1)
• Tested applications: FC, IP, RIA, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was generated by immunizing female BALB/c mice with whole Raji cells or a crude membrane preparation.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD74
• UniProt ID: P04233
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ii; p33; DHLAG; HLADG; Ia-GAMMAHLA; HLA Class II h
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Daudi cells with anti-CD74 LL1 (EPB1). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of LL1 (EPB1) (orb1089962) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089962, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD74 antibody LL1 (EPB1). Human spleen(A) (0.01 µg/ml) and human tonsil(B) (0.05 ug/ml) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of LL1 (EPB1) (orb1089962) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.