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CD70 Biosimilar Antibody
Catalog Number: orb689310
| Catalog Number | orb689310 |
|---|---|
| Category | Antibodies |
| Description | Anti-CD70 (vorsetuzumab biosimilar) mAb |
| Target | CD70 |
| Clonality | Monoclonal |
| Species/Host | Human |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Powder |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| UniProt ID | P32970 |
| Tested applications | ELISA, FC |
| Dilution range | ELISA 1:5000-10000 Flow Cyt 1:100 |
| Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Antibody Type | Biosimilar Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | CD70CD27LCD27LGTNFSF7 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD70, mFc-His tagged protein orb689401 can bind Anti-CD70 Neutralizing antibody in a linear range of 3.91-139.9 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human CD70 (A) were surface stained with anti-CD70 neutralizing antibody 1 μg/ml (vorsetuzumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
Flow cytometry analysis of antigen binding of anti-human CD70 mAb (orb689310). (A) A clear peak shift of orb689310 was seen compared to the control when incubated with CD70-expressing THP1 cells, indicating strong binding of orb689310 to CD70. Antibodies were incubated at 2 μg/mL.
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