Anti-CD70 [TAN1-6]

• Catalog Number: orb758813
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD70
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: TAN1-6
• Tested applications: Blocking, FC, IF, WB
• Reactivity: Mouse
• Isotype: Rat IgG
• Immunogen: This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD70
• UniProt ID: O55237
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFSF7; CD27 ligand; CD27-L; Cd27l; CD27LG; CD70 a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD70 antibody TAN1-6. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of TAN1-6 (orb758813, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TAN1-6 (orb758813) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758813, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody