Anti-CD7 [3A1E]

• Catalog Number: orb613801
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD7
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3A1E
• Tested applications: FA, FC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunizing BALB/c mice with CD7-expressing HSB-2 T-cells
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD7
• UniProt ID: P09564
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leu9; T-cell antigen CD7; GP40; T-cell leukemia an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody 3A1E. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3A1E (orb613801) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613801, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD7 antibody 3A1E Jurkat cell lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 3A1E (orb613801) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa, but this protein is glycosylated at several residues. orb613801 successfully detected CD7 in Jurkat cell lysate.