Anti-CD64 [H22]

• Catalog Number: orb613911
• Category: Antibodies
• Description: Human monoclonal antibody to CD64
• Target: CD64
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Human IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA'􏳑).
• UniProt ID: P12314
• Tested applications: ELISA, FC
• Clone Number: H22
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-High affinity immunoglobulin gamma Fc recepto
• Note: For research use only

Immunofluorescence staining of fixed U937 cells with anti-CD64 antibody H22. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of H22 (orb613912) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613912, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.