Anti-CD63 [NK-1-C3]

• Catalog Number: orb758930
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD63
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NK-1-C3
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD63
• UniProt ID: P08962
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Granulophysin; LAMP-3; Tetraspanin-30; Tspan-30; L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of NK-1-C3 (orb758931) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758931, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD63 antibody NK-1-C3 K562 cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of NK-1-C3 (orb758931) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.